Light Microscopy

• Introduction to Modern Methods in Light Microscopy.- Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM).- Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy.- Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis.- Imaging of Brain Slices with a Genetically Encoded Voltage Indicator.- FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells.- Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum.- Targeted Ablation Using Laser Nanosurgery.- Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells.- STED Imaging in Drosophila Brain Slices.- Two-Color Total Internal Refection Fluorescence Microscopy of Exocytosis in Endocrine Cells.- Optical Coherence Microscopy.- Designing Image Analysis Pipelines in Light Microscopy: A Rationale Approach.- Automated Analysis of Intracellular Dynamic Processes.- Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells.- Analysis of Protein Kinetics using Fluorescence Recovery After Photobleaching (FRAP).- Fluorescence Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening.

“This well-illustrated book provides up-to-date light microscopy approaches and tool sets offered for live or fixed cell observations with a focus on system instrumentation parameters and sophisticated labeling and detection methods. … authors hope that this book will prove to be a robust teaching guide for light microscopy practical courses. I echo the authors’ opinion. … an excellent book which may serve as a user manual on current light microscopy approaches and toolsets for live or fixed cell observations.” (Omer Iqbal, Doody's Book Reviews, July, 2017)