Del 4 - Methods in Molecular Biology

New Nucleic Acid Techniques

Inbunden, Engelska, 1988

Av John M. Walker, John M Walker

2 749 kr

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Produktinformation

Utgivningsdatum1988-08-31
Mått155 x 235 x 32 mm
Vikt1 216 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor560
Upplaga1988
FörlagHumana Press Inc.
ISBN9780896031272

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in ?gt10 or ?gt11.- Immunoscreening of ?gt11 Expression Libraries Using an Avidin–Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.-Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts.

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