Light Microscopy

• Glycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model.- Histological processing of teeth and periodontal tissues for light microscopy analysis.- Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding?.- Image cytometry: nuclear and chromosomal DNA quantification.- Histological approaches to study tissue parasitism during Trypanosoma cruzi infection.- Intravital microscopy to study leukocyte recruitment in vivo.- Introduction to fluorescence microscopy.- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals.- Imaging lipid bodies within leukocytes with different light microscopy techniques.- EicosaCell – An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites.- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer.- Imaging calcium sparks in cardiac myocytes.- Light microscopy in aquatic ecology: methods for plankton communities studies.- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples.

From the reviews:“The book is superbly illustrated with the great majority of the figures in colours and is certainly a must not only for beginners … but I am pretty sure that even the practised colleagues in doing every day use of microscopic methods will find very useful tricks … . thanks to this well planned and clearly written book we are stimulated to imagine new applications.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)