• Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library.- Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast.- Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo.- Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease.- Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA).- Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility.- Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination.- An Efficient Purification System for Native Minichromosome from S. cerevisiae.- Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit.- Analysis of Stable and Transient Protein-Protein Interactions.- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP).- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS).- Analysis of Chromatin Structure in Plant Cells.- Analysis of Histones and Histone Variants in Plants.- Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays.- A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers.- In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA.- Activator-Dependent Acetylation of Chromatin Model Systems.- Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates.- Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET).- Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure.- Sulfyhydryl Reactive Site-Directed Crosslinking as a Method for Probing the Tetrameric Structure of Histones H3 and H4.- Genomic Approaches for Determining Nucleosome Occupancy in Yeast.- Genome-Wide Approaches to Determining Nucleosome Occupancy in Metazoans Using MNase-Seq.- Salt Fractionation of Nucleosomes for Genome-Wide Profiling.- Quantitative Analysis of Genome-Wide Chromatin Remodeling.- Computational Analysis of Nucleosome Positioning.

From the reviews:“This is an outstanding and timely collection of methods concerning how one can investigate chromatin remodelling. … There are detailed laboratory procedures and notes attached to all chapters, and this enable readers to use the volume as a laboratory manual. … The editor, Randall Morse, has to be congratulated on producing such an excellent book; a book that I recommend most highly to researchers in this arena.” (Raymond Waters, Human Genetics, Vol. 131, 2012)

• Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library.- Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast.- Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo.- Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease.- Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA).- Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility.- Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination.- An Efficient Purification System for Native Minichromosome from S. cerevisiae.- Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit.- Analysis of Stable and Transient Protein-Protein Interactions.- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP).- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS).- Analysis of Chromatin Structure in Plant Cells.- Analysis of Histones and Histone Variants in Plants.- Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays.- A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers.- In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA.- Activator-Dependent Acetylation of Chromatin Model Systems.- Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates.- Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET).- Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure.- Sulfyhydryl Reactive Site-Directed Crosslinking as a Method for Probing the Tetrameric Structure of Histones H3 and H4.- Genomic Approaches for Determining Nucleosome Occupancy in Yeast.- Genome-Wide Approaches to Determining Nucleosome Occupancy in Metazoans Using MNase-Seq.- Salt Fractionation of Nucleosomes for Genome-Wide Profiling.- Quantitative Analysis of Genome-Wide Chromatin Remodeling.- Computational Analysis of Nucleosome Positioning.

From the reviews:“This is an outstanding and timely collection of methods concerning how one can investigate chromatin remodelling. … There are detailed laboratory procedures and notes attached to all chapters, and this enable readers to use the volume as a laboratory manual. … The editor, Randall Morse, has to be congratulated on producing such an excellent book; a book that I recommend most highly to researchers in this arena.” (Raymond Waters, Human Genetics, Vol. 131, 2012)

• Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library.- Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast.- Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo.- Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease.- Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA).- Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility.- Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination.- An Efficient Purification System for Native Minichromosome from S. cerevisiae.- Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit.- Analysis of Stable and Transient Protein-Protein Interactions.- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP).- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS).- Analysis of Chromatin Structure in Plant Cells.- Analysis of Histones and Histone Variants in Plants.- Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays.- A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers.- In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA.- Activator-Dependent Acetylation of Chromatin Model Systems.- Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates.- Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET).- Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure.- Sulfyhydryl Reactive Site-Directed Crosslinking as a Method for Probing the Tetrameric Structure of Histones H3 and H4.- Genomic Approaches for Determining Nucleosome Occupancy in Yeast.- Genome-Wide Approaches to Determining Nucleosome Occupancy in Metazoans Using MNase-Seq.- Salt Fractionation of Nucleosomes for Genome-Wide Profiling.- Quantitative Analysis of Genome-Wide Chromatin Remodeling.- Computational Analysis of Nucleosome Positioning.

From the reviews:“This is an outstanding and timely collection of methods concerning how one can investigate chromatin remodelling. … There are detailed laboratory procedures and notes attached to all chapters, and this enable readers to use the volume as a laboratory manual. … The editor, Randall Morse, has to be congratulated on producing such an excellent book; a book that I recommend most highly to researchers in this arena.” (Raymond Waters, Human Genetics, Vol. 131, 2012)