Xenopus

This detailed volume brings together protocols spanning classical and modern approaches to using Xenopus laevis and/or Xenopus tropicalis in scientific research. From maintaining healthy colonies and raising tadpoles to protocols for embryo culture, microinjection, CRISPR and base editing, and transgenesis, this book showcases the versatility of this tractable model in the study of developmental biology, organogenesis, human disease, and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Xenopus: Methods and Protocols serves as an ideal guide for researchers both new to, and familiar with, this adaptable model organism.Chapters 1, 3, 9, 11, and 20 are available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.