Vaccine Delivery Technology

Methods and Protocols

Häftad, Engelska, 2020

AvBlaine A. Pfeifer,Andrew Hill

1 849 kr

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This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes.

Produktinformation

Utgivningsdatum2020-09-22
Mått178 x 254 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor590
FörlagSpringer-Verlag New York Inc.
ISBN9781071607978

Tillhör följande kategorier

Onkologi inom Medicin
Kemi inom Naturvetenskap och teknik
Biokemisk teknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Maskinteknik och material inom Naturvetenskap och teknik
Farmakologi inom Medicin
Naturvetenskap:allmänt inom Naturvetenskap och teknik

Vaccine Delivery and Immune Response Basics.- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.- Antigen Discovery in Bacterial Pan-Proteomes.- Alphavirus-Based Antigen Preparation.- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.- Production of Zika Virus Virus-Like Particles.- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.- Whole-Cell Vaccine Preparation: Options and Perspectives.- O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein.- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.- Attenuation Methods for Live Vaccines.- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.- Lipofection-Based Delivery of DNA Vaccines.- Flavivirus DNA Vaccine Design and Adjuvant Selection.- Vaccine Delivery with a Detoxified Bacterial Toxin.- Needle-Less or Non-Invasive Delivery Technology.- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.- A Hybrid Biological-Biomaterial Vector for Antigen Delivery.- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot.- The Measurement of Antigen-Specific IgG Titers by Direct ELISA.- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.

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