Del 1291 i serien Methods in Molecular Biology

T follicular Helper Cells

Methods and Protocols

Häftad, Engelska, 2016

1 579 kr

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This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.

Produktinformation

Utgivningsdatum2016-10-05
Mått178 x 254 x 13 mm
Vikt514 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor228
FörlagHumana Press Inc.
ISBN9781493955930

Tillhör följande kategorier

Cellbiologi inom Naturvetenskap och teknik

Identification of Mouse T Follicular Helper Cells by Flow Cytometry.- Identification of Follicular T Helper Cells in Tissue Sections.- Tracking Early T Follicular Helper Cell Differentiation In vivo.- Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination.- Retroviral Vector Expression in TCR Transgenic CD4+ T Cells.- Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells.- In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function.- Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation.- The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses.- Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205.- Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry.- Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry.- In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses.- Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells.- Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential.- Analysis of Human Blood Memory T Follicular Helper Subsets.- Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood.- Quantifying Helper Cell Function of Human TFH Cells in vitro.