Structural Biology of Membrane Proteins

• Part One: Expression and Purification of Membrane Proteins;Chapter 1: Refolding of G-protein-coupled receptors;1: Introduction;2: Refolding of membrane proteins;3: In vitro protein refolding;4: GPCR in vitro refolding;5: Conclusion;6: References;Chapter 2: Expression of genes encoding eukaryotic membrane proteins in mammalian cells;1: Introduction;2: Mammalian cell hosts and gene expression vectors;3: Delivery and maintenance of expression vectors in mammalian host cells;4: Use of HEK293S stable cell lines for high-level expression of eukaryotic membrane proteins;5: Large-scale growth of HEK293S stable cell lines in suspension culture using a bioreactor and inducible gene expression;6: Preparation of eukaryotic membrane proteins containing simple N-glycans;7: Outlook for use of HEK293S tetracycline-inducible cell lines for large-scale preparation of other eukaryotic membrane proteins;Chapter 3: Expression of recombinant G-protein-coupled receptors for structural biology;1: Introduction;2: Expression of recombinant GPCRs;3: Conclusions;4: References;Chapter 4: The purification of G-protein-coupled receptors for crystallization;1: Introduction;2: Heterogeneity of overexpressed receptors;3: Membrane fractionation, solubilization and detergent selection;4: Purification;5: Final quality control, monitoring protein stability, aggregational state, lipid and bound detergent;6: Conclusions;Chapter 5: An introduction to detergents and their use in membrane protein studies;1: Introduction;2: Physical properties of detergents used in membrane protein studies;3: Extraction and purification procedure using common detergents;4: Use of detergents in membrane protein crystallization;5: Conclusion;6: References;Part Two: Methods for Structural Characterization of Membrane Proteins;Chapter 6: Solution NMR approaches to the structure and dynamics of integral membrane proteins;1: Introduction;2: Protein production and optimization for NMR studies;3: NMR methodology for the study of integral membrane proteins;4: Solution NMR structures of helical integral membrane proteins;5: Solution NMR Structures of ?-barrel membrane proteins;6: Solution NMR characterization of membrane protein dynamics;7: Future directions;8: References;Chapter 7: Membrane proteins studied by solid-state NMR;1: Introduction;2: Sample preparation and methodology;3: Applications;4: Conclusions;5: References;Chapter 8: Assessing structure and dynamics of native membrane proteins;1: Introduction;2: Assembly of 2D crystals;3: Electron microscopy;4: Atomic force microscopy;5: Conclusion and perspectives;Chapter 9: State-of-the-art methods in electron microscopy, including single particle analysis;1: Introduction;2: Sample Preparation;3: Low Dose Microscopy;4: Applications of CryoEM;5: Examples;6: Conclusion;Chapter 10: Atomic resolution structures of integral membrane proteins using cubic lipid phase crystallization;1: Introduction;2: Membrane protein crystals & crystallization;3: Advantages of structures in a native setting at high resolution;4: Conclusions;5: References;Part Three: New Membrane Protein Structures;Chapter 11: Aquaporins: Integral membrane channel proteins;1: Introduction;2: Aquaporin channel proton exclusion barrier;3: Selectivity in the aquaporin family;4: Permeation by substances other than water and glycerol;5: Aquaporin monomer associations and their functional implications;6: References;Chapter 12: Gas channels for ammonia;1: Introduction;2: The structure of Ammonia Channel;3: Reconstituted into liposomes AmtB acts as a channel that conducts NH3;4: The Mechanism of conduction;5: The Rh proteins;6: Comparison with AQPs;7: Comparison with K+ channel;8: Acknowledgement;9: References;Chapter 13: Channels in the outer membrane of Mycobacter;1: Introduction;2: Structure determination;3: Structure description;4: The outer membrane;5: Conclusion;6: References;Chapter 14: The structure of the SecY protein translocation channel;1: Abstract;2: Introduction;3: Structure determination of the SecY complex by electron cryo-microscopy;4: Determination of the X-ray crystal structure of the SecY complex;5: Description of the structure of the SecY complex;6: Post-translational translocation in bacteria;7: Conclusions and outlook;8: References;Chapter 15: Structure and function of the translocator domain of bacterial autotransporters;1: Introduction;2: The NalP autotransporter;3: The translocator domain of autotransporters;4: Purification and in vitro folding of the NalP translocator domain;5: The structure of the NalP translocator domain;6: Comparison of the NalP translocator domain to other translocator domains and to TolC;7: The autotransporter secretion mechanism;Chapter 16: X-ray crystallographic structures of sarcoplasmic reticulum Ca2+-ATPase at the atomic level;1: Introduction;2: The transport scheme and thermodynamics of Ca2+ transport;3: Overall structure of Ca2+-ATPase;4: Transport models;5: Initialization of the cycle - phosphorylation and calcium ion occlusion;6: The dephosphorylation step and proton counter transport;7: Getting Ca2+ in and out of the membrane;8: Compact versus open conformations of SERCA;9: Conclusions;Chapter 17: Comparison of the multidrug transporter EmrE structures determined by electron cryo-microscopy and X-ray crystallography;1: Introduction;2: The oligomeric state of EmrE;3: Transport activity of EmrE;4: Structure of EmrE determined by electron cryomicroscopy;5: Comparison of the EmrE structure determined by electron crystallography with a 3.8 + resolution structure determined by X-ray crystallography;6: Conclusion;7: References;Chapter 18: Structure of photosystems I and II;1: Introduction to oxygenic photosynthesis;2: Photosystem ii;3: Photosystem I;4: Conclusion and outlook;Chapter 19: Glutamate receptor ion channels - Structural insights into molecular mechanism;1: Introduction;2: Studies of the Ligand-binding Domain;3: The Functional Architecture of a Glutamate Receptor Ion Channel;4: A Working Model of AMPA Receptor Function;5: Open Questions;6: References;Chapter 20: The mitochondrial ADP/ATP carrier;1: Introduction;2: Mitochondrial carriers and ADP/ATP carrier;3: Crystallization;4: Diffraction, phasing and model building;5: Structure analysis;6: Functional implications;7: Future developments and conclusions;

...an up-to-date account of the state of the art in this field....a snapshot of the state of the art in the structural biology of membrane proteins.This book fills a gap and can serve both the membrane-protein researcher who is interested in other methods as well as other scientists who wish to get a general overview of the current state of membrane-protein structural biology.A very well presented and informative account of structural biology of membrane proteins.