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In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.
Plant Tissue Cultures.- Computerized Molecular Modelling of Carbohydrates.- Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS.- High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors.- Carbohydrate Gel Electrophoresis.- Capillary Electrophoresis with Detection by Laser-Induced Fluorescence.- Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls.- Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays.- Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls.- Analyzing Cellulose Biosynthesis with Confocal Microscopy.- Visual Mapping of Cell Wall Biosynthesis.- Atomic Force Microscopy of Plant Cell Walls.- Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls.- Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation.- Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis.- New Insights into the Control of Cell Growth.- Extraction and Detection of Arabinogalactan Proteins.- Characterization of the Plant Cell Wall Proteome Using High Throughput Screens.- Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens.- Measuring In vitro Extensibility of Growing Plant Cell Walls.
