Overview I.- Methods for Studying Embryo Implantation and Uterine Biology.- Methodologies to Study Implantation in Mice.- Blastocyst Culture.- Isolation of Hormone Responsive Uterine Stromal Cells.- Rat Decidual Cell Cultures.- The Immortalization of Human Endometrial Cells.- Sheep Uterine Gland Knockout (UGKO) Model.- A Baboon Model for Inducing Endometriosis.- A Baboon Model for Simulating Pregnancy.- The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research.- In Vitro Trophoblast and Placental Model Systems.- Mouse Trophoblast Stem Cells.- Connexins and Trophoblast Cell Lineage Development.- Rcho-1 Trophoblast Stem Cells.- Bovine Trophoblast Cell Culture Systems.- In Vitro Induction of Trophoblast from Human Embryonic Stem Cells.- Isolation and Culture of Term Human Trophoblast Cells.- Production of Human Trophoblast Cell Lines.- Culture and Transfection of Human Choriocarcinoma Cells.- In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion.- Phenotypic Analysis of the Placenta.- Phenotypic Analysis of the Mouse Placenta.- Phenotypic Analysis of the Rat Placenta.- Analysis of the Structure of the Ruminant Placenta.- Characterization of the Bovine Placenta by Cytoskeleton, Integrin Receptors, and Extracellular Matrix.- Molecular Markers for Human Placental Investigation.- Correlative Microscopy of Ultrathin Cryosections in Placental Research.- Vascular Corrosion Casting of the Uteroplacental and Fetoplacental Vasculature in Mice.- Analysis of Fetal and Maternal Microvasculature in Ruminant Placentomes by Corrosion Casting.- Molecular Analysis and Gene Transfer Techniques.- Microarray Analysis of Trophoblast Cells.- Gene Expression Microarray Data Analysis of Decidual and Placental Cell Differentiation.- Assays to Determine Allelic Usage of Gene Expression in the Placenta.- Adenoviral-Mediated Gene Delivery to Trophoblast Cells.
"...provides step-by-step instructions for conducting research in mammalian embryology." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal "...an important contribution to the literature...recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal