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In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty.
Placenta and Trophoblast: Methods and Protocols: Overview II.- Analysis of Trophoblast Invasion.- In Vivo Analysis of Trophoblast Cell Invasion in the Human.- In Vitro Analysis of Trophoblast Invasion.- An In Vitro Model of Trophoblast Invasion of Spiral Arteries.- Analysis of Uteroplacental Immune Cells and Their Functions.- In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells.- Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface.- Methods for Isolation of Cells from the Human Fetal-Maternal Interface.- In Vitro Models for Studying Human Uterine and Placental Macrophages.- Macrophage-Trophoblast Interactions.- Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon.- Analysis of the Soluble Isoforms of HLA-G.- Analysis of Placental Function.- In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport.- In Vitro Models for Studying Trophoblast Transcellular Transport.- In Vitro Methods for Studying Human Placental Amino Acid Transport.- In Vitro Methods for Studying Human Placental Amino Acid Transport.- Methods for Investigating Placental Fatty Acid Transport.- Heterologous Expression Systems for Studying Placental Transporters.- Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures.- Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells.- Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands.- Bacterial Expression of Prolactin Family Proteins.- Analysis of Placental Regulation of Hematopoiesis.- Methods for Studying Interferon Tau Stimulated Genes.- ANALYSIS OF PLACENTA ADAPTATION TO DISEASE.- Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia.- In Vivo Rat Model of Preeclampsia.- A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice.- Working with Oxygen and Oxidative Stress In Vitro.- Hypobaric Hypoxia as a Tool to Study Pregnancy-Dependent Responses at the Maternal-Fetal Interface.- Infection With Listeria monocytogenes as a Probe for Placental Immunological Function.
"...will provide an excellent laboratory resource for years to come." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal "...an important contribution to the literature, and I recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal