Nanotoxicity

• Historical Overview of Nanotechnology and Nanotoxicology.- Characterization of Nanomaterials for Toxicological Studies.- Methods for Understanding the Interaction Between Nanoparticles and Cells.- Single-Cell Gel Electrophoresis (COMET) Assay in Nano-Genotoxicology.- Single-Cell Nanotoxicity Assays of Superparamagnetic Iron Oxide Nanoparticles (SPIONs).- Western Blot Analysis.- Application of Reverse Transcription-PCR and Real-Time PCR in Nanotoxicity Research.- Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase (LDH) Activity Indirectly.- Enzyme-Linked Immunosorbant Assay of IL-8 Production in Response to Silver Nanoparticles.- Metabolomics Techniques in Nanotoxicology Studies.- Nanoparticle Uptake Measured by Flow Cytometry.- Determining Biological Activity of Nanoparticles as Measured by Flow Cytometry.- Whole Cell Impedance Biosensoring Devices.- Free Energy Calculation of Permeant-Membrane Interactions Using Molecular Dynamics Simulations.- Screening of Fullerene Toxicity by Haemolysis Assay.- Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model.- In Vivo Methods of Nanotoxicology.- The Luminescent Bacteria Test to Determine the Acute Toxicity of Nanoparticle Suspensions.- The Primacy of Physicochemical Characterization of Nanomaterials for Reliable Toxicity Assessment: A Review of the Zebrafish Nanotoxicology Model.- Application of Embryonic and Adult Zebrafish for Nanotoxicity Assessment.- Applications of Subsurface Microscopy.- Application of ICP-MS for the Study of Disposition and Toxicity of Metal-Based Nanomaterials.- Quantitative Nanoparticle Organ Disposition by Gel Permeation Chromatography.- Physiologically-Based Pharmacokinetic Modeling For Nanoparticle Toxicity Study.- Biophysical Methods for Assessing Plant Responses to Nanoparticle Exposure.- In Vivo Nanotoxicity Assays in Plant Models.

From the reviews:“A practical guide for scientists from different disciplines, including toxicologists, material scientists, cell biologists and pharmaceutical scientists. … Undoubtedly, the book contains a wealth of useful nanotoxicity research protocols. … Nanotoxicity: Methods and Protocols provides a wealth of practical information and experimental protocols related to the increasingly relevant topic of Nanotoxicity and it will be useful to researchers across many disciplines.” (Driton Vllasaliu, BTS Newsletter – The British Toxicology Society, Issue 43, Winter, 2013)