Membrane Transporters

• Pharmacogenomics of Membrane Transporters.- The IUBMB-Endorsed Transporter Classification System.- Bioinformatics and Data Integration in Membrane Transporter Studies.- DNA Microarrays to Analyze Gene Expression in Animals with Altered Transporter Expression.- Microarray Data Analysis in Studies of Membrane Transporters.- Laser Capture Microdissection to Examine Transporter Expression in Specific Cell Regions.- Site-Directed Mutagenesis in the Study of Membrane Transporters.- Fluorescence Techniques for Studying Membrane Transport Proteins.- Reconstitution of Membrane Transporters.- Equilibrium Binding and Transport Studies.- Electrophysiological Studies of Ion Channels and Therapeutic Potential With Adenovirus-Mediated Gene Transfer.- Antibody Production and Immunocytochemical Localization of Amino Acid Transporters.- Xenopus laevis Oocytes.- Measurement of Intracellular pH.- Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domains.- Membrane Protein NMR Studies.- Analysis of Soluble Sugar Permease Domains by Solution NMR.- Molecular Modeling and Molecular Dynamics Simulations of Membrane Transporter Proteins.- Imaging Plasma Membrane Proteins by Confocal Microscopy.

"The book is a useful reference to have for bench scientists. The protocols provide a good starting point for someone seeking technical advice for various procedures used to study transport."-Doody's Health Sciences Book Review Journal "...a valuable laboratory or office protocols book that combines, distils and updates traditional and novel bench and in silico techniques for the study of membrane transporters." - Clinical and Experimental Pharmacology and Physiology