Del i serien Methods in Molecular Biology

Membrane Transporters

Methods and Protocols

Inbunden, Engelska, 2003

1 379 kr

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This collection of cutting-edge methodologies for studying membrane transporters and channels takes advantage of all the latest developments in biomedical research, including pharmacogenomcs, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics studies, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microcopy are also described, along with several that may help in the development of novel therapeutics.

Produktinformation

Utgivningsdatum2003-05-02
Mått155 x 235 x 28 mm
Vikt694 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor372
Upplaga2003
FörlagHumana Press Inc.
ISBN9781588291042

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Pharmacogenomics of Membrane Transporters.- The IUBMB-Endorsed Transporter Classification System.- Bioinformatics and Data Integration in Membrane Transporter Studies.- DNA Microarrays to Analyze Gene Expression in Animals with Altered Transporter Expression.- Microarray Data Analysis in Studies of Membrane Transporters.- Laser Capture Microdissection to Examine Transporter Expression in Specific Cell Regions.- Site-Directed Mutagenesis in the Study of Membrane Transporters.- Fluorescence Techniques for Studying Membrane Transport Proteins.- Reconstitution of Membrane Transporters.- Equilibrium Binding and Transport Studies.- Electrophysiological Studies of Ion Channels and Therapeutic Potential With Adenovirus-Mediated Gene Transfer.- Antibody Production and Immunocytochemical Localization of Amino Acid Transporters.- Xenopus laevis Oocytes.- Measurement of Intracellular pH.- Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domains.- Membrane Protein NMR Studies.- Analysis of Soluble Sugar Permease Domains by Solution NMR.- Molecular Modeling and Molecular Dynamics Simulations of Membrane Transporter Proteins.- Imaging Plasma Membrane Proteins by Confocal Microscopy.

"The book is a useful reference to have for bench scientists. The protocols provide a good starting point for someone seeking technical advice for various procedures used to study transport."-Doody's Health Sciences Book Review Journal "...a valuable laboratory or office protocols book that combines, distils and updates traditional and novel bench and in silico techniques for the study of membrane transporters." - Clinical and Experimental Pharmacology and Physiology