Laboratory Manual of Microbiology

A.K. Roy: Professor and Head, Course Coordinator, P.G. Department of Biotechnology, T.M. Bhagalpur, University, Bhagalpur, Bihar,India.M.M. Prasad: Professor, University Department of Botany, T.M. Bhagalpur, University, Bhagalpur, Bihar,India

• 1. Organization of microbiology laboratory. 2. Preparation of media-liquid and solid for growth of microorganisms. 3. Isolation techniques and maintenance of microorganisms. 4. Isolation of pure cultures from soil, air, water and seed surface. 5. Staining and microscopic examination of microbes. 6. Micrometry and measurement of spores. 7. Microbial growth. 8. Study of mutations by Ames test. 9. Assay of antibiotics, antibiotics sensitivity of microbes and demonstration of antibiotic resistance. 10. Bacterial transformation. 11. Biochemical characterization of enteric bacteria (IMVIC test). 12. Isolation and one step growth curve of coliphage. 13. Testing of water quality (bacteriological examination of water). 14. Culture from body fluids (urine, stool, blood). 15. Mixed acid and alcoholic fermentation.