Immunotoxicity Testing

• Immunotoxicology Testing: Past and Future.- Overview and Health-Risk Considerations.- Developmental Immunotoxicity (DIT): The Why, When, and How of DIT Testing.- An In Vivo Tiered Approach to Test Immunosensitization by Low Molecular Weight Compounds.- Risk of Autoimmune Disease: Challenges for Immunotoxicity Testing.- Markers of Inflammation.- Evaluating Macrophages in Immunotoxicity Testing.- Immunotoxicity and Host Resistance Models.- Host Resistance Assays Including Bacterial Challenge Models.- Viral Host Resistance Studies.- Parasite Challenge as Host Resistance Models for Immunotoxicity Testing.- Tumor Challenges in Immunotoxicity Testing.- Testing Protocols in Rodents and Other Laboratory Animals.- The T-Dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents.- The Sheep Erythrocyte T-Dependent Antibody Response (TDAR).- The Delayed Type Hypersensitivity Assay Using Protein and Xenogeneic Cell Antigens.- The Cytotoxic T Lymphocyte Assay for Evaluating Cell-Mediated Immune Function.- NK Cell Assays in Immunotoxicity Testing.- The Local Lymph Node Assay and Skin Sensitization Testing.- Use of Contact Hypersensitivity in Immunotoxicity Testing.- Evaluation of Apoptosis in Immunotoxicity Testing.- Dendritic Cells in Immunotoxicity Testing.- Evaluating Cytokines in Immunotoxicity Testing.- Flow Cytometry in Preclinical Drug Development.- Enhanced Histopathology Evaluation of Lymphoid Organs.- Immunotoxicity Testing in Nonhuman Primates.- Evaluation in Humans.- Fundamentals of Clinical Immunotoxicology.- Wildlife Testing.- In Vivo Functional Tests for Assessing Immunotoxicity in Birds.- In Vitro Alternatives.- In Vitro Testing for Direct Immunotoxicity: State of the Art.

From the reviews:“This volume forms part of the Springer Protocols ‘Methods in Molecular Biology’ series … . All the chapters are quite well written, being concise and clearly laid out. … In conclusion, this is a useful laboratory professional’s ‘workshop manual’ for the most well-established immunotoxicity assays, focusing very much on the procedures at the laboratory bench. It will be a useful basic source of information for anyone starting to establish some of the test methods in their own laboratory.” (Peter R. Ryle, BTS Newsletter, Issue 37, Winter, 2010)“In this book, Dr. Dietert of Cornell University has done an excellent job in covering concepts of immunotoxicology associated with analytical approaches to investigate mechanisms of toxicity and for the diagnosis of poisoning. Through very well-devised and illustrated texts by renowned researchers in immunotoxicology, the 26 chapters of this book cover the vast literature on approaches and strategies for immunotoxic testing, with detailed laboratory protocols. Thus, the book serves as a reference source for students, laboratory technicians and scientific researchers in toxicology.” (Sandra Farsky, Brazilian Journal of Pharmaceutical Sciences, May, 2010)“This book provides a collection of testing approaches and strategies with detailed laboratory protocols in the successful style of the Methods in Molecular Biology series. … audience includes not only scientists working directly in the field of immunotoxicology, but also students, technicians, and lab and safety office personnel. … meant to be used by a specific subgroup of immunologists, the protocols are also used in many laboratory situations and are not limited to immunotoxicity studies. … The book appears to fill an important niche.” (Marion C. Cohen, Doody’s Review Service, September, 2010)