Helicobacter pylori Protocols

• Introduction: Medical Significance of H. pylori.- Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture.- Serology and Urea Breath Test in the Diagnosis of H. pylori Infection.- The Detection of H. pylori by the Polymerase Chain Reaction.- PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples.- In vitro Susceptibility Testing of H. pylori.- Defined Media for H. pylori.- Studies of Respiration in H. pylori.- Use of NMR to Study H. pylori Metabolism.- Isolation of H. pylori Genomic DNA and Restriction Analysis.- Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori.- Differentiation of H. pylori Strains Using PCR RFLP.- PCR-DNA Sequence Typing.- H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method.- Molecular Methods for Detecting Ulcerogenic Strains of H. pylori.- H. pylori DNA Transformation by Natural Competence and Electroporation.- Random Mutagenesis of the H. pylori Genome.- Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE).- Molecular Characterization of H. pylori Surface Antigens.- Isolation and Characterization of Porins from H. pylori.- Methods for the Identification of H. pylori Host Receptors.- Host Responses to H. pylori.- Nonprimate Animal Models of H. pylori Infection.- The Nonhuman Primate Model for H. pylori Infection.

"The book was designed to provide up-to-date detailed protocols for the experienced either working in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine