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A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies - PCR, RIA, ELISA, and latex-agglutination - that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Rapid Detection of Adenovirus from Fecal Specimens.- Alphaviruses.- Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR.- Enteroviruses and Rhinoviruses.- Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction.- Hepatitis Viruses.- Herpesviruses.- Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper.- PCR for the Detection of Influenza Viruses in Clinical Material.- Lyssaviruses.- Methods for Detecting Antimeasles, Mumps, and Rubella Virus Antibodies.- Papillomaviruses.- Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization.- Polioviruses.- Poxviruses.- Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections.- Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis.- Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNA by Calorimetric Enzymatic Immunoassay.- Time-Resolved Fluorescence.- Enzyme-Linked Immunosorbent Assays.- Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology.- Robotic Analyzers.- The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction.- Recombinant Antigens in Viral Diagnosis.- NASBA.- Oligonucleotides.
"...an invaluable source of information for clinical and non-clinical virologists and microbiologists...would be a good asset to most research laboratories...For those that do perform these assays, it is invaluable."-Doody's Health Sciences Book Review Journal
