Handbook of ELISPOT

• Unique Strengths of ELISPOT for T Cell Diagnostics.- The Impact of Harmonization on ELISPOT Assay Performance.- Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells.- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes.- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay.- Dual and Triple Color Fluorospot.- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes.- ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells.- ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion.- Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell.- How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity.- Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay.- Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles.- Statistical Analysis of ELISPOT Assays.- Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories.- Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT.- ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis.- IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®.- Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays.

From the reviews of the second edition: "In this second edition ... the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. ... This text would be key reading material for any laboratory planning to set up this technique. ... this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple." (Hayley Evans, Immunology News, November, 2012)