Handbook of ELISPOT

• Unique Strengths of ELISPOT for T Cell Diagnostics.- The Impact of Harmonization on ELISPOT Assay Performance.- Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells.- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes.- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay.- Dual and Triple Color Fluorospot.- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes.- ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells.- ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion.- Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell.- How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity.- Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay.- Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles.- Statistical Analysis of ELISPOT Assays.- Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories.- Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT.- ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis.- IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®.- Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays.

From the reviews of the second edition:“In this second edition … the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. … This text would be key reading material for any laboratory planning to set up this technique. … this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple.” (Hayley Evans, Immunology News, November, 2012)