Fundamentals of Recombinant Protein Production, Purification and Characterization

Dr. Deepti Yadav grew up in Lucknow, Uttar Pradesh, India. After getting a master’s degree in microbiology from Delhi University, she joined the Durban University of Technology in South Africa to pursue her PhD in biotechnology with Prof. Tukayi Kudanga’s research group within the Department of Biotechnology and Food Science.Dr. Yadav has worked in a number of research labs, where she gained hands-on experience in techniques such as molecular biology, tissue culture, cloning, protein expression in recombinant hosts and protein purification. She is particularly interested in the fields of enzyme biotechnology, enzyme kinetics, and enzyme applications.After obtaining her PhD degree, Dr. Yadav was hired as a Research Associate by the School of Energy Materials at Mahatma Gandhi University, Kottayam, where she is working on synthesis of polyhydroxybutyrate from a locally available water hyacinth plant. She has written several scholarly papers and has been invited to speak at conferences. She has also coached and encouraged numerous young students to seek professions in biotechnology.Dr. Abhishek Guldhe is currently working as Associate Professor, Amity institute of Biotechnology, Amity University Maharashtra, Mumbai. He was awarded with prestigious Ramalingaswami Re-entry Fellowship of Department of Biotechnology, Government of India in 2019. He has featured in the list of “World’s top 2% scientists” published by Stanford University, USA. His research focus area is microalgal biotechnology, bioactive compounds, and waste beneficiation. He has worked in many national and multinational funded projects with local governing bodies, industry, and international research groups. He has presented his work in international conferences and workshops in India, South Africa, Brazil, USA, and Canada. Prof. Tukayi Kudanga holds a PhD in biotechnology and is a professor in the Department of Biotechnology and Food Science at Durban University of Technology, South Africa. He is a recognized authority in laccase applications, particularly in biocatalysis and surface functionalization of lignocellulose materials. His areas of expertise include enzyme biotechnology, biocatalysis, and biomaterials. His current research focuses on enzymatic synthesis of bioactive compounds, enzymatic modification of food biopolymers, and development of green technologies for functionalization of lignocellulose materials. He is also interested in the search for novel biocatalysts, developing new applications and modifying enzymes for relevant biotechnological applications. Prof. Kudanga has extensive experience in teaching and research and has published his works widely. He has been invited to be plenary speaker at international conferences and reviews for several scientific journals.

• 1. Introduction to recombinant protein expression and purification2. Recombinant protein expression in Escherichia coli3. Recombinant protein expression in Pichia pastoris4. Mammalian cells for recombinant protein production via transient gene transfer5. Expression of recombinant proteins in Chlamydomonas reinhardtii6. Extraction and concentration of proteins (Laboratory scale)7. Chromatographic and non-chromatographic techniques for purifying recombinant proteins8. Analysis for purity of recombinant proteins9. Characterization of recombinant proteins