Combinatorial Biotechnology

Dr. Preeti Srivastava obtained PhD from Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi in the area of corynebacterial plasmid biology. She did postdoctoral research work in the area of chromosome dynamics in Vibrio cholerae from Laboratory of Biochemistry and molecular biology, National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, Maryland, USA. She has worked as Senior Scientist in Environmental Biotechnology Division, Indian Institute of Toxicology Research (CSIR), Lucknow before joining IIT Delhi in 2011. Currently, she is working as Professor, Department of Biochemical Engineering and Biotechnology, IIT Delhi. Her present research interest is in Bacterial genetics and Environmental Biotechnology. She has published several papers in Internationally reputed journals and proceedings of National and International conferences. She has been granted 4 patents and has filed three patent applications. She is the recipient of the prestigious Innovative Young Biotechnologist Award and TATA innovation fellowship from Department of Biotechnology, Ministry of Science and technology, Government of India.

• 1. Solid Phase synthesis2. Solid phase peptide synthesis3. Solid phase Oligonucleotide synthesis4. Solid phase oligosaccharide synthesis5. Parallel peptide synthesis on solid phase Parallel synthesis: microplate method, Tea bag approach, Multi pin approach, SPOT synthesis, Light directed parallel synthesis, parallel synthesis using micro fluidic devices, peptide synthesis using peptide laser printer, cLIFT6. Split only approach for peptide synthesis7. Split and mix approach for peptide synthesis8. Encoding Technologies Peptide encoding, Shaving, Oligonucleotide encoding, PNA encoding, Binary encoding, Mass encoding, optical encoding using fluorescent colloids, internal encoding, Physical encoding, electronic encoding, Graphical encoding.9. Solution phase synthesis Dendrimers, Polymer supported reagents, scavenger resins, Fluorous reactants10. Encoding in solution phase11. Deconvolution methods Iterative deconvolution, Positional scanning, Orthogonal deconvolution, Subtractive deconvolution, Bogus coin deconvolution, Recursive deconvolution12. Chemical Ligations Native Chemical ligation, Intein mediated ligation, Staudinger ligation13. Biological methods: In vivo Display Libraries Filamentous phage display, lambda phage display, Bacterial cell surface display, Yeast cell surface display, Baculovirus capsid display, Mammalian cell surface display14. Biological methods: In vitro display libraries Ribosomal display, mRNA display, CIS/CAD display, Plasmid display, In vitro compartmentalization15. Genetic code reprogramming