Environmental Responses in Plants

Paula DuquePlant Molecular Biology groupInstituto Gulbenkian de CiênciaRua da Quinta Grande, 62780-156 OeirasPortugalPhone: +351 214464630Fax: +351 214407970Email: duquep@igc.gulbenkian.pt

• Hydrotropism: Analysis of the Root Response to a Moisture Gradient.- Assessing Gravitropic Responses in Arabidopsis.- Physiological Analysis of Phototropic Responses in Arabidopsis.- Automatic Chloroplast Movement Analysis.- Microscopic and Biochemical Visualization of Auxins in Plant Tissues.- Immunolocalization of PIN and ABCB Transporters in Plants.- Analysis of Circadian Leaf Movements.- Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes.- A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are Temporally Restricted by the Circadian Clock.- Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome.-  Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.- Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock.- Assessing Protein Stability Under Different Light and Circadian Conditions.- Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.- Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana.- Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedlings.- Assessing Drought Responses Using Thermal Infrared Imaging.- Generating Targeted Gene Knockout Lines in Physcomitrella Patens to Study Evolution of Stress-Responsive Mechanisms.- Screening Stress Tolerance Traits in Arabidopsis Cell Cultures.- Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress.- Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum.- Virus-Induced Gene Silencing for Gene Function Studies in Barley.- Methods for Long-Term Stable Storage of Colletotrichum Species.- Plant Inoculation With the Fungal Leaf Pathogen Colletotrichum higginsianum.- Tracing Plant Defense Responses in Roots Upon MAMP/DAMP Treatment.- Analysis of the Immunity-Related Oxidative Bursts by a Luminol-Based Assay.- Quantitative Analysis of Microbe- Associated-Molecular-Pattern (MAMP)-Induced Ca2+ Transients in Plants.- Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR.- Determining Nucleosome Position at Individual Loci after Biotic Stress Using MNase-qPCR.- Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis thaliana.