Ebolaviruses

• Marburg and Ebola Viruses: A Look Back and Lessons for the Future.- 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies.- Ebolavirus: An Overview of Molecular and Clinical Pathogenesis.- Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms.- Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins.- Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.- Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation.- Modeling Ebolavirus Budding with Virus-Like Particles.- Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays.- Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins.- Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA.- A Semi-Automated High Throughput Microtitration Assay for Filoviruses.- Generation of Recombinant Ebola Viruses Using Reverse Genetics.- Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals.- Live-Cell Imaging of Filoviruses.- Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds.- Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence.- Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells.- Electron Microscopy of Ebola Virus-Infected Cells.- Validating the Inactivation Effectiveness of Chemicals on Ebola Virus.- Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS).- Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection.- Evaluation of Ebola Virus Countermeasures in Guinea Pigs.- Evaluation of Medical Countermeasures against Ebolaviruses in Non-Human Primate Models.- Quantification of Filovirus Glycoprotein-Specific Antibodies.- Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR.- Real-Time and End Point PCR Diagnostics for Ebola Virus.- Production of Antigens for ELISA.- ELISA Methods for the Detection of Ebolavirus Infection.- Ebola Virus Field Sample Collection.

“This book comprises three review chapters on filoviruses and 27 protocol chapters. … Scientists working on filoviruses and those wishing to enter the field will find a compendium of carefully written methods and protocols relevant to their work. … What makes the book stand out from many other volumes in this series is the inclusion of the three review chapters, which by themselves earn the book a spot on the bookshelf of any scientist working in the field.” (Christoph Wirblich, Doody's Book Reviews, January, 2018)