DNA Methylation

• DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker.- Global Methylation Levels.- Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry.- Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels.- Methods For Whole Genome Analysis Of Dna Methylation Patterns.- Immunodetection Array.- Methylated DNA Immunoprecipitation (MeDIP).- The MIRA Method for DNA Methylation Analysis.- The Help Assay.- Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray.- Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS).- Methylation-Sensitive Representational Difference Analysis (MS-RDA).- Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis.- GoldenGate® Assay for DNA Methylation Profiling.- 5?-Azacytidine Expression Arrays.- Gene-Specific Methylation Analysis.- DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones.- Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology.- Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods.- Melting Curve Assays for DNA Methylation Analysis.- Methylation SNaPshot: A Method for the Quantification of Site-Specific DNA Methylation Levels.- Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips.- Restriction Digestion and Real-Time PCR (qAMP).- MethylQuant: A Real-Time PCR-Based Method to Quantify DNA Methylation at Single Specific Cytosines.- Methylation-Specific PCR.- MethyLight.- Quantification of Methylated DNA by HeavyMethyl Duplex PCR.-Special Applications.- Analysis of Methylated Circulating DNA in Cancer Patients’ Blood.- Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA.- Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification.

From the reviews of the second edition: "The 2nd edition ... resulted in an extraordinarly helpful tool for lab practice and for developing multiples approaches in order to successfully perform these type of analysis. ... authors made each chapter a valuable tool for beginners and senior investigators who want to explore DNA methylation analysis in their biological systems. ... used for advanced researchers in order to complement their work with other methodologies. ... This is definitively a volume that must be present at each lab working in this cutting-edge field ... ." (Marcia Manterola, European Journal of Histochemistry, October, 2009)