Clostridium difficile

Methods and Protocols

Häftad, Engelska, 2018

1 419 kr

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This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile.

Produktinformation

Utgivningsdatum2018-06-09
Mått178 x 254 x 17 mm
Vikt576 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor292
Upplaga2
FörlagHumana Press Inc.
ISBN9781493981762

Tillhör följande kategorier

Onkologi inom Medicin

Restriction Endonuclease Analysis Typing of Clostridium Difficile Isolates.- Direct PCR-ribotyping of Clostridium difficile.- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data.- Clostridium difficile Genome Editing Using pyrE Alleles.- Use of mCherryOpt Fluorescent Protein in Clostridium difficile.- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile.- Clostridium difficile Adhesins.- Intestinal Epithelial Cell Response to Clostridium difficile Flagella.- Isolating and Purifying Clostridium difficile Spores.- Inducing and Quantifying Clostridium difficile Spore Formation.- Characterization of Functional Prophages in Clostridium difficile.- Induction and Purification of C. difficile Phage Tail-like Particles.- Phage Transduction.- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics.- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile.- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains.- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection.- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community.- A Practical Method for Preparation of Fecal Microbiota Transplantation.- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine.- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins.