Del i serien Methods in Molecular Biology

Clostridium difficile

Methods and Protocols

Häftad, Engelska, 2016

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Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide.

Produktinformation

Utgivningsdatum2016-08-23
Mått178 x 254 x 14 mm
Vikt482 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor241
FörlagHumana Press Inc.
ISBN9781493957019

Tillhör följande kategorier

to Clostridium difficile and the Disease It Causes.- Clostridium difficile: No Longer an Enigmatic Pathogen?.- Clostridium difficile and the Disease It Causes.- Isolation and Culture Techniques.- Clostridium difficile Isolation and Culture Techniques.- Methods for Typing and Epidemiological Studies.- Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping.- Clostridium difficile Toxinotyping.- Multilocus Sequence Typing for Clostridium difficile.- Biochemistry of the Organism.- Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes.- Dissecting the Cell Surface.- Human Intestinal Epithelial Response(s) to Clostridium difficile.- Genomics.- Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays.- Development of Systems for Genetic Analysis of the Organism.- ClosTron-Targeted Mutagenesis.- Methods for Gene Cloning and Targeted Mutagenesis.- Transposon Mutagenesis in Clostridium difficile.- Animal Models of Disease.- Refinement of the Hamster Model of Clostridium difficile Disease.- Methods for Working with the Mouse Model.

From the reviews:“This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea after prolonged antibiotic treatment. … This book would be useful to scientists working with bacteria such as C. difficile that cause toxin-mediated diseases in the colon of their host. … This is a technical book that provides essential methods and techniques to study devastating diseases due to Clostridium difficile.” (Rebecca T. Horvat, Doody’s Review Service, October, 2010)