Chlamydia trachomatis

Methods and Protocols

Häftad, Engelska, 2020

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This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis.

Produktinformation

Utgivningsdatum2020-08-14
Mått178 x 254 x 17 mm
Vikt576 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor289
FörlagHumana Press Inc.
ISBN9781493996964

Tillhör följande kategorier

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA).- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue.- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae.- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay.- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth.- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples.- Multi Locus Sequence Typing (MLST) of Chlamydiales.- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method.- Dual RNA-Seq of Chlamydia and Host Cells.- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection.- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions.- Mutagenesis of Chlamydia trachomatis Using TargeTron.- Forward and Reverse Genetic Analysis of Chlamydia.- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions.- Rodent Infections for Chlamydia spp..- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy.- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane.- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis.