bokomslag Chlamydia trachomatis
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Chlamydia trachomatis

Amanda Claire Brown

Inbunden

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  • 289 sidor
  • 2019
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
  • Författare: Amanda Claire Brown
  • Format: Inbunden
  • ISBN: 9781493996933
  • Språk: Engelska
  • Antal sidor: 289
  • Utgivningsdatum: 2019-08-05
  • Förlag: Humana Press Inc.