Del i serien Methods in Molecular Biology

Chemogenomics

Methods and Protocols

Häftad, Engelska, 2024

2 029 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays.

Produktinformation

Utgivningsdatum2024-08-11
Mått178 x 254 x 14 mm
Vikt474 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor234
FörlagSpringer-Verlag New York Inc.
ISBN9781071633991

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Farmakologi inom Medicin

An Introduction to Chemogenomics.- Developing a Kinase Chemogenomic Set: Facilitating Investigation into Kinase Biology by Linking Phenotypes to Targets.- Compilation of Custom Compound/Bioactivity Datasets from Public Repositories.- Quality Control of Chemogenomic Library using LC-MS.- Annotation of the Effect of Chemogenomic Compounds on Cell Health using High-Content Microscopy in Live-Cell Mode.- Characterization of Cellular Viability using Label-Free Brightfield Live-Cell Imaging.- Plate-Based Screening for DUB Inhibitors.- NanoBRET™ Live Cell Kinase Selectivity Profiling Adapted for High Throughput Screening.- A Fluorescence-Based Reporter Gene Assay to Characterize Nuclear Receptor Modulators.- Measuring Protein-Protein Interactions in Cells using NanoLuciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay.- HiBiT Cellular Thermal Shift Assay (HiBiT CETSA).- Detection of Cellular Target Engagement for Small Molecule Modulators of Striatal-Enriched Protein Tyrosine Phosphatase (STEP).- Target Deconvolution by Limited Proteolysis Coupled to Mass Spectrometry.- Global Assessment of Drug Target Engagement and Selectivity of Covalent Cysteine-Reactive Inhibitors using Alkyne-Functionalized Probes.- 3D-Spheroid Invasion Assay for High-Throughput Screening of Small Molecule Libraries.- Live-Cell High-Throughput Screen for Monitoring Autophagy Flux.- Phenotypic Chemical Screening in CD4+ T Cells to Identify Epigenetic Inhibitors.