Cell Biology Assays

A native of Chile, Dr. Julio E. Celis received his bachelor’s degree from the University of Chile in 1964, and then completed his Ph.D. in the United States at the University of Iowa. After his postdoctoral training at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England, he went on to become an assistant professor at the University of Chile. Dr. Celis was named an associate professor in 1975 at Aarhus University, Denmark, and in 1987 became Chairman of the University’s Institute of Medical Biochemistry. He is also the Chairman of the Danish Centre for Human Genome Research and was recently elected Secretary General-elect of the Federation of European Biochemical Societies (FEBS) 1997. With nearly 180 publications to his credit, Dr. Celis’ specialized areas of research include molecular mechanisms of cancer, human 2D gel protein databases and their link to genome data, signal transduction, and the biology of human skin.Married, with three children and one cat (Max William), Dr. Celis currently resides in Denmark where he holds among his many titles the chair to the Symposium and Prize Committee of the Danish Cancer Society, as well as the acting Chairman of the Nordic Molecular Biology Association (NOMBA). Dr. Celis is currently the Vice President of the European Molecular Biology Laboratory (EMBL) Council and leader of the Danish Delegation to the OECD Megascience Forum subgroup on Bioinformatics. He is also the European Union Observer to the International Nucleotide sequence database collaboration and a member of the EMBO Course Committee.

• Protein DeterminationPhosphopeptide Mapping: A Basic ProtocolCoupling of Fluorescent Tags to ProteinsRadioiodination of Proteins and PeptidesFree-Flow ElectrophoresisGel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome AnalysisTwo-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological SamplesAffinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood PlasmaImage Analysis and QuantitationFluorescence Detection of Proteins in Gels Using SYPRO DyesAutoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat SamplesTwo-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope LabelingImmunoprecipitation of Proteins under Nondenaturing ConditionsNondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation ComplexesAffinity purification with Natural Immobilized LigandsAnalysis of Protein-Protein Interactions by Chemical Cross-LinkingPeroxisomal Targeting as a Tool to Assess Protein-Protein InteractionsBiomolecular Interaction Analysis Mass SpectrometryBlot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction SitesLigand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding ProteinsModular Scale Yeast Two-Hybrid ScreeningChromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite GreenChromatin Immunoprecipitation (ChIP)Gel Mobility Shift AssayDNA Affinity Chromatography of Transcription Factors: The Oligaonucleotide Trapping ApproachProtein Degradation Methods: Chaperone-Mediated AutophagyMethods in Protein UbiquitinationProtein Identification and Sequencing by Mass SpectrometryProteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ionization Mass SpectrometryIn-Gel Digestion of Protein Spots for Mass SpectrometryPeptide Sequencing by Tandem Mass SpectrometryDirect Database Searing Using Tandem Mass Spectra of PeptidesIdentification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching ToolsIdentification of Protein Phosphorylation Sites by Mass SpectrometryAnalysis of Carbohydrates/Glycoproteins by Mass SpectrometryStable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative ProteomicsSite-Specific, Stable Isotopic Labeling of Cysteinyl Pepties in Complex Peptide MixturesProtein Hydrogen Exchange measured by Electrospry Ionization Mass SpectrometryNongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide MixturesMass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies