Cell Biology Assays

A native of Chile, Dr. Julio E. Celis received his bachelor’s degree from the University of Chile in 1964, and then completed his Ph.D. in the United States at the University of Iowa. After his postdoctoral training at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England, he went on to become an assistant professor at the University of Chile. Dr. Celis was named an associate professor in 1975 at Aarhus University, Denmark, and in 1987 became Chairman of the University’s Institute of Medical Biochemistry. He is also the Chairman of the Danish Centre for Human Genome Research and was recently elected Secretary General-elect of the Federation of European Biochemical Societies (FEBS) 1997. With nearly 180 publications to his credit, Dr. Celis’ specialized areas of research include molecular mechanisms of cancer, human 2D gel protein databases and their link to genome data, signal transduction, and the biology of human skin.Married, with three children and one cat (Max William), Dr. Celis currently resides in Denmark where he holds among his many titles the chair to the Symposium and Prize Committee of the Danish Cancer Society, as well as the acting Chairman of the Nordic Molecular Biology Association (NOMBA). Dr. Celis is currently the Vice President of the European Molecular Biology Laboratory (EMBL) Council and leader of the Danish Delegation to the OECD Megascience Forum subgroup on Bioinformatics. He is also the European Union Observer to the International Nucleotide sequence database collaboration and a member of the EMBO Course Committee.

• Permeabilized Epithelial Cells to Study Exocytic Membrane TransportStudying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based ApproachesAssays Measuring Membrane Transport in the Endocytic PathwayMicrosome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian CellsCotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian CellsUse of Permeabilsed Mast Cells to Analyze Regulated ExocytosisSyringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell LoadingCell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial MonolayersProtein Translocation into MitochondriaPolarographic Assays of Mitochrondrial FunctionsAnalysis of Nuclear Protein Import and Export in Digitonin-Permeabilized CellsHeterokaryons: An Assay for Nucleocytoplasmic ShuttlingDNA Replication-Dependent Chromatin Assembly SystemCygnets: Intracellular Guanosine 3’,5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy TransferCa2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)Ratiometric PericamFuorescent Indicators for Imaging Protein Phosphorylation in Single Living CellsIn Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular ProteinsDissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional CommunicationDetection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent ProteinsTracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiaeMicrotubule Motility Assays In vitro Assays for Mitotic Spindle Assembly and FunctionIn vitro Motility Assays with ActinUse of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria monocytogenesPedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin CytoskeletonListeria monocytogenes: Techniques to Analyze Bacterial Infection in vitroMeasurement of Cellular Contractile Forces Using Patterned Elastomer