Advanced Technologies for Protein Complex Production and Characterization

Volume II

Inbunden, Engelska, 2024

Av M. Cristina Vega, Francisco J. Fernández, Francisco J. Fernandez

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This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes.

Produktinformation

Utgivningsdatum2024-03-21
Mått178 x 254 x 18 mm
Vikt686 g
FormatInbunden
SpråkEngelska
SerieAdvances in Experimental Medicine and Biology
Antal sidor213
FörlagSpringer International Publishing AG
ISBN9783031521928

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

Dr. M. Cristina Vega leads a research group at the Chemical and Structural Biology Department, part of the Center for Biological Research Margarita Salas (CIB-CSIC) in Madrid, Spain. Her research blends biochemical, biophysical, and structural biology techniques to explore the mechanisms by which pathogens circumvent the human complement system and other immune defenses and to develop innovative diagnostic and therapeutic approaches. Dr. Francisco J. Fernández, as the CEO of Abvance Biotech SL, a cutting-edge biopharmaceutical company in Madrid, Spain, brings his extensive expertise in structural biology and recombinant protein production and characterization to the forefront. His work is distinguished by a profound interest in applying synthetic and computational biology tools and techniques for therapeutic antibody development. Dr. Fernández’s innovative approach in these fields underpins his leadership in advancing biotechnological applications in medicine.

Chapter 1: Immunoprecipitation methods to isolate messenger ribonucleoprotein complexes (mRNP).- Chapter 2: Purification of in vivo or in vitro-assembled RNA-protein complexes by RNA centric methods.- Chapter 3: Peptide-based mass spectrometry for protein complexes investigation.- Chapter 4: Probing protein complexes composition, stoichiometry, and interactions by peptide-based mass spectrometry.- Chapter 5: Discovery and examination of linear motif mediated protein-protein complexes.- Chapter 6: Protein-protein binding kinetics by biolayer interferometry.- Chapter 7: Studying Macromolecular Interactions of Cellular Machines by the Combined use of Analytical Ultracentrifugation, Light Scattering, and Fluorescence Spectroscopy Methods.- Chapter 8: The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions.- Chapter 9: X-ray crystallography for protein complexes.- Chapter 10: Characterizationof biological samples using ultra-short and ultra-bright XFEL pulses.- Chapter 11: Small-angle X-ray scattering for macromolecular complexes.- Chapter 12: Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.- Chapter 13: Characterization of Complexes and Supramolecular Structures by Electron Microscopy.