This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization.

Produktinformation

Utgivningsdatum2024-03-21
Mått178 x 254 x 18 mm
Vikt686 g
FormatInbunden
SpråkEngelska
SerieAdvances in Experimental Medicine and Biology
Antal sidor213
FörlagSpringer International Publishing AG
ISBN9783031521928

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

Dr. M. Cristina Vega leads a research group at the Chemical and Structural Biology Department, part of the Center for Biological Research Margarita Salas (CIB-CSIC) in Madrid, Spain. Her research blends biochemical, biophysical, and structural biology techniques to explore the mechanisms by which pathogens circumvent the human complement system and other immune defenses and to develop innovative diagnostic and therapeutic approaches. Dr. Francisco J. Fernández, as the CEO of Abvance Biotech SL, a cutting-edge biopharmaceutical company in Madrid, Spain, brings his extensive expertise in structural biology and recombinant protein production and characterization to the forefront. His work is distinguished by a profound interest in applying synthetic and computational biology tools and techniques for therapeutic antibody development. Dr. Fernández’s innovative approach in these fields underpins his leadership in advancing biotechnological applications in medicine.

Chapter 1: Immunoprecipitation methods to isolate messenger ribonucleoprotein complexes (mRNP).- Chapter 2: Purification of in vivo or in vitro-assembled RNA-protein complexes by RNA centric methods.- Chapter 3: Peptide-based mass spectrometry for protein complexes investigation.- Chapter 4: Probing protein complexes composition, stoichiometry, and interactions by peptide-based mass spectrometry.- Chapter 5: Discovery and examination of linear motif mediated protein-protein complexes.- Chapter 6: Protein-protein binding kinetics by biolayer interferometry.- Chapter 7: Studying Macromolecular Interactions of Cellular Machines by the Combined use of Analytical Ultracentrifugation, Light Scattering, and Fluorescence Spectroscopy Methods.- Chapter 8: The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions.- Chapter 9: X-ray crystallography for protein complexes.- Chapter 10: Characterizationof biological samples using ultra-short and ultra-bright XFEL pulses.- Chapter 11: Small-angle X-ray scattering for macromolecular complexes.- Chapter 12: Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.- Chapter 13: Characterization of Complexes and Supramolecular Structures by Electron Microscopy.

Mer från samma serie

Mechanisms of Lymphocyte Activation and Immune Regulation

Sudhir Gupta, William E. Paul, Anthony Fauci

Häftad, 2012

1 439 kr

Frontiers in Modeling and Control of Breathing

Chi-Sang Poon, Homayoun Kazemi, Chi-Sang Poon, Homayoun Kazemi

Inbunden, 2001

2 969 kr

Biology of Taurine

Ryan J. Huxtable, F. Franconi, A. Giotti

Inbunden, 1987

2 869 kr

Molecular Immunology of Complex Carbohydrates

Albert M. Wu, L. Garry Adams

Inbunden, 1988

1 439 kr

Mechanisms of Physical and Emotional Stress

George P. Chrousos, D. Lynn Loriaux, Philip W. Gold

Inbunden, 1989

2 959 kr

Del 281

Fibrinogen, Thrombosis, Coagulation and Fibrinolysis

Chung Yuan Liu, Chung Yuan Liu, Shu Chien

Inbunden, 1991

1 189 kr

Molecular and Cellular Biology Prostate Cancer

Symposium on Molecular and Cellular Biol, James P. Karr, etc.

Inbunden, 1991

1 189 kr

Nutritional and Toxicological Consequences of Food Processing

Mendel Friedman

Inbunden, 1991

2 959 kr

Recombinant Technology in Haemostasis and Thrombosis

Leon W. Hoyer, Leon Ed Hoyer, W.N. Drohan, Leon W. Hoyer, W N Drohan, Leon W Hoyer

Inbunden, 1991

1 189 kr

New Aspects of Human Polymorphonuclear Leukocytes

W.H. Hörl, P.J. Schollmeyer

Inbunden, 1991

2 959 kr

Du kanske också är intresserad av

Del 1453

Advanced Technologies for Protein Complex Production and Characterization

M. Cristina Vega, Francisco J. Fernández

Häftad, 2025

2 449 kr

Del 1453

Multipotent Stem Cells of the Hair Follicle

Robert M Hoffman

Inbunden, 2016

1 449 kr

Del 1453

Advancements in VLSI Design, Microelectronics Devices and Circuits

Pradeep Kumar, William Navaraj, Fernando Moreira

Inbunden, 2025

4 029 kr

Del 1453

Multipotent Stem Cells of the Hair Follicle

Robert M Hoffman

Häftad, 2018

1 369 kr

Del 1453

Das Geschenk der Weisen

O. Henry

Inbunden, 2018

279 kr

Del 1453

Verhaeltnis Der Eg-Fusionskontrollverordnung Zu Artikel 85 Und 86 Des Ewg-Vertrages

Stefan Kurz

Häftad, 1993

849 kr

Del 1453

AI Applications in Cyber Security and Privacy of Communication Networks

Chaminda E.R. Hewage, Mohammad Haseeb Zafar, Nishtha Kesswani

Häftad, 2025

3 259 kr

Del 1453

Advances in Computational Intelligence Systems

Nitin Naik, Paul Jenkins, Paul Grace, Longzhi Yang, Shaligram Prajapat

Häftad, 2024

2 969 kr

Del 896

Advanced Technologies for Protein Complex Production and Characterization

M. Cristina Vega

Inbunden, 2016

2 669 kr

Del 1453

Global Analysis - Studies and Applications IV

Yurii G. Borisovich, Yurii E. Gliklikh

Häftad, 1990

509 kr

Del 1453 i serien Advances in Experimental Medicine and Biology

Advanced Technologies for Protein Complex Production and Characterization

Volume II

Inbunden, Engelska, 2024

AvM. Cristina Vega,Francisco J. Fernández

2 449 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

Finns i fler format (3)

Häftad

2 449 kr

Skickas inom 10-15 vardagar

Inbunden

2 669 kr

Skickas inom 10-15 vardagar

Häftad

2 589 kr

Skickas inom 10-15 vardagar

Produktinformation

Utgivningsdatum2024-03-21
Mått178 x 254 x 18 mm
Vikt686 g
FormatInbunden
SpråkEngelska
SerieAdvances in Experimental Medicine and Biology
Antal sidor213
FörlagSpringer International Publishing AG
ISBN9783031521928

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

Chapter 1: Immunoprecipitation methods to isolate messenger ribonucleoprotein complexes (mRNP).- Chapter 2: Purification of in vivo or in vitro-assembled RNA-protein complexes by RNA centric methods.- Chapter 3: Peptide-based mass spectrometry for protein complexes investigation.- Chapter 4: Probing protein complexes composition, stoichiometry, and interactions by peptide-based mass spectrometry.- Chapter 5: Discovery and examination of linear motif mediated protein-protein complexes.- Chapter 6: Protein-protein binding kinetics by biolayer interferometry.- Chapter 7: Studying Macromolecular Interactions of Cellular Machines by the Combined use of Analytical Ultracentrifugation, Light Scattering, and Fluorescence Spectroscopy Methods.- Chapter 8: The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions.- Chapter 9: X-ray crystallography for protein complexes.- Chapter 10: Characterizationof biological samples using ultra-short and ultra-bright XFEL pulses.- Chapter 11: Small-angle X-ray scattering for macromolecular complexes.- Chapter 12: Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.- Chapter 13: Characterization of Complexes and Supramolecular Structures by Electron Microscopy.