Multipotent Stem Cells of the Hair Follicle

Robert M. Hoffman, Ph.D.Professor of Surgery, University of California, San DiegoPresident, AntiCancer, Inc. San Diego, CA, USA.

• Introduction to Hair-Follicle-AssociatedPluripotent (HAP) Stem Cells.- Nestin-Based Reporter Transgenic Mouse Lines.- Discovery of HAP Stem Cells.- Peripheral-Nerve and Spinal-Cord Regeneration inMice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells.- Constructionof Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair FollicleNeural Crest Stem Cells.- Nestin-ExpressingHair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term3D Gelfoam Histoculture.- Isolation and Culture of Neural Crest StemCells from Human Hair Follicles.- Isolation of Mouse Hair Follicle Bulge Stem Cellsand their Functional Analysis in a ReconstitutionAssay.- Hair Follicle Regeneration by Transplantationof a Bioengineered Hair Follicle Germ.- Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation.- Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.- Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.- Isolation and Fluorescence Activated Cell-Sorting ofMouse Keratinocytes Expressing β-Galactosidase.- Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cordof Mice.- Long-Term Gelfoam® Histoculture ofHair-Shaft-Producing Mouse Whisker Follicles.- From Hair to Heart: Hair Follicle-Associated-Pluripotent(HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells.- Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium.- Cryopreservation of Intact Hair Follicle Maintains Pluripotencyof Nestin-Expressing (HAP) Stem Cells.