Accurate Results in the Clinical Laboratory

Amitava Dasgupta received his Ph. D in chemistry from Stanford University and completed his fellowship training in Clinical Chemistry from the Department of Laboratory Medicine at the University of Washington School of Medicine at Seattle. He is board certified in both Toxicology and Clinical Chemistry by the American Board of Clinical Chemistry. Currently, he is a tenured Full Professor of Pathology and Laboratory Medicine at the University of Kansas Medical Center and Director of Clinical Laboratories at the University of Kansas Hospital. Prior to this appointment he was a tenured Professor of Pathology and Laboratory Medicine at the University of Texas McGovern medical School from February 1998 to April 2022. He has 252 papers to his credit. He is in the editorial board of four journals including Therapeutic Drug Monitoring, Clinica Chimica Acta, Archives of Pathology and Laboratory Medicine, and Journal of Clinical Laboratory Analysis.Jorge Sepulveda received his M.D. from the University of Lisbon, Portugal, and his residency training in Laboratory Medicine and Ph.D. degree in Cell and Molecular Biology from Baylor College of Medicine, Houston, Texas. He is board certified by the American Board of Pathology in Clinical Pathology and Transfusion Medicine. He has published over 35 research articles, review papers and book chapters, and serves as Associate Editor for the Archives of Pathology and Laboratory Medicine. Dr. Sepulveda has broad experience in laboratory medicine as medical director of various clinical laboratories, including at the Houston and Philadelphia Veterans Affairs Medical Centers and at the University of Pittsburgh Presbyterian and Shadyside Hospitals, and currently serves as Associate Medical Director of the Clinical Laboratories at the Columbia University Medical Center Campus of New York-Presbyterian Hospital.

• Part I: Sources of errors in clinical laboratories and how to eliminate such errors?1 Variation, errors, and quality in the clinical laboratory2 Errors in patient preparation, specimen collection, anticoagulant and preservative use: How to avoid such pre-analytical errors.3 Sample processing and specimen misidentification issues: Major sources of pre-analytical error4 Patient-Related Factors Influencing Clinical Laboratory Test Results and Interpretation5 Interferences of hemolysis, lipemia and high bilirubin on Laboratory Tests6 Immunoassay design7 Overview of other sources of interferences in immunoassays: Prozone effect and interferences from heterophilic antibodies and autoantibodies8 Biotin interference in clinical laboratory tests: New Solutions to an Evolving ProblemPart II: Sources of errors in clinical chemistry laboratory9 Challenges in Routine Clinical Chemistry Testing: Analysis of Small Molecules10 Challenges in Routine Clinical Chemistry Analysis of Proteins and Enzymes11 Challenges in endocrinology testing12 Pitfalls in Testing for Common Tumor MarkersPart III: Sources of errors in therapeutic drug monitoring and toxicology13 Issues of Interferences in Therapeutic Drug Monitoring14 Limitations of immunoassays for screening of drugs of abuse in urine due to issues of interferences (cross-reactivities) : a new interpretive paradigm15 Confirmation of Immunoassay Drug Screens: From Structural Similarity to Molecular Identity16 Issues of False Negative Results in Toxicology: Difficult in Detecting Certain Drugs and Issues with Detection of Synthetic Cathinone (Bath Salts), Synthetic Cathinone (Spice), and Other New Psychoactive Substances17 Alcohol testing in the clinical laboratory: considerations for optimal use.Part IV: Herbal medicines and laboratory testings18 Effects of Herbal Supplements on Clinical Laboratory Test ResultsPart V: Sources of errors in immunology laboratory19 Critical Issues in Hemoglobin Variant Testing20 Sources of errors in immunology and serology testingPart VI: Sources of errors in molecular, genetic and related testing21 Sources of Error in Molecular Diagnostic Analyses22 Molecular Testing for Targeted Therapies and Pharmacogenomics23 Challenges and Sources of Inaccuracy in Biochemical Genetics TestingPart VII: Sources of errors in microbiology testing24 Sources of pre-analytical, analytical and post-analytical errors in the clinical microbiology laboratory25 Accuracy Considerations in Molecular Microbiology: Sources of Errors26 Microbiology and the Health System at Large: Best Practices for Effective Use of ResultsPart VIII: Sources of errors in hematology and coagulation testing27 Sources of Errors in Hematology Testing28 Sources of Errors in Coagulation Testing29 Sources of errors in flow cytometrySection IX: Sources of errors in blood banking and transfusion medicine30 Interferences in Blood Typing, Compatibility Testing and Serological Testings31 Molecular testing in transfusion medicine32 Errors in Transfusion Medicine and Transfusion ReactionsPart X: Sources of errors in point of care testing33 Methodological issues in point of care testing devices34 Special Concern: Sources of Inaccuracy in Breath Alcohol AnalysisPart XI: Informatics35 Avoiding common pitfalls in clinical laboratory informatics