Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish,  applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and  the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.  Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomplement to the first book for new and experienced zebrafish researcheralike.