Zebrafish

Methods and Protocols

Inbunden, Engelska, 2023

Av James F. Amatruda, Corinne Houart, Koichi Kawakami, Kenneth D. Poss

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This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology.

Produktinformation

Utgivningsdatum2023-09-06
Mått178 x 254 x 26 mm
Vikt903 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor360
Upplaga3
FörlagSpringer-Verlag New York Inc.
ISBN9781071634004

Tillhör följande kategorier

Delivering Traumatic Brain Injury to Larval Zebrafish.- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish.- Methods to Study Liver Disease Using Zebrafish Larvae.- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening.- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ).- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions.- Primary Culture of Neuronal Populations for Various Downstream Applications.- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish.- Brain Imaging and Registration in Larval Zebrafish.- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy.- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish.- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows.- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette.- Spinal Cord Injury and Assays for Regeneration.- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase.- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins.- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein.- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones.- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish.- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression.- Scalable CRISPR Screens in Zebrafish Using MIC-Drop.- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.