• Approaches and Species in the History of Vertebrate Embryology.- Manipulating and Imaging the Early Xenopus laevis Embryo.- Manipulation of Gene Function in Xenopus laevis.- Developmental Genetics in Xenopus tropicalis.- Embryological and Genetic Manipulation of Chick Development.- Embryological Manipulations in Zebrafish.- Practical Approaches for Implementing Forward Genetic Strategies in Zebrafish.- Essential Techniques for Introducing Medaka to a Zebrafish Laboratory - Toward the Combined Use of Medaka and Zebrafish for Further Genetic Dissection of the Function of the Vertebrate Genome.- Ex-utero Culture and Imaging of Mouse Embryos.- Detection of Gene Expression in Mouse Embryos and Tissue Sections.- Gene Targeting in the Mouse.- Creating a “Hopeful Monster”: Mouse Forward Genetic Screens.- Assisted Reproductive Technology in Nonhuman Primates.- Embryological Methods in Ascidians: the Villefranche-sur-Mer Protocols.- Ciona Genetics.- Analyses of Gene Function in Amphioxus Embryos by Microinjection of mRNAs and Morpholino-oligonucleotides.- Reptile Embryology.- Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii.- Mutant Generation in Vertebrate Model Organisms by TILLING.- Inducing High Rates of Targeted Mutagenesis in Zebrafish using Zinc Finger Nucleases (ZFNs).- Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic Cell Nuclear Transfer.- Cloning Mice and ES Cells by Nuclear Transfer from Somatic Cells and Fully Differentiated Cells.- Keeping Two Animal Systems in One Lab: A Frog Plus Fish Case Study.- Laboratory Guidelines for Animal Care.

From the reviews:“Prof. Francisco J. Pelegrini … deserves the great merit of assembling a twenty-four chapters volume that will surely help researchers and educators thanks to both the choice of the topics covered (which includes even a chapter dealing with the laboratory guidelines for animal care) and to the easy-to-follow protocols that highlight each chapter.” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 56, 2012)