Vascular Biology Protocols

• Rat Carotid Artery Balloon Injury Model.- Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model.- Plaque Rupture Model in Mice.- Immunostaining of Mouse Atherosclerotic Lesions.- Surgical Animal Model of Ventricular Hypertrophy.- Animal Models of Hypertension.- Rat Models of Cardiac Insulin Resistance.- The Isolated, Perfused Pseudo-Working Heart Model.- Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice.- Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress.- Detection and Quantification of Apoptosis in the Vasculature.- Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction.- Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells.- Evaluation of Endothelial Function by In Vivo Microscopy.- Haptotaxis of Endothelial Cell Migration Under Flow.- Isolation, Culture, and Functional Analysis of Adult Mouse Cardiomyocytes.- Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes.- Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia.- Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells.- Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues.- Assessment of Protein Glycoxidation in Ventricular Tissues.- Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes.- Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research.- Stem Cell Therapy in the Heart and Vasculature.

From the reviews: "This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models. ... Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. ... This would be a valuable addition to any laboratory doing research in vascular biology." (Vera P. Shively, Doody's Review Service, December, 2008)