This detailed volume covers new and classic methods for vaccinia virus (the prototype poxvirus) but also extends to methods and protocols specifically tailored for mpox virus, lumpy skin disease virus, and myxoma virus. As these viruses continue to expand their territories, research into the impact of poxviruses on human and animal health, as well as investigating vaccine vectors and oncolytic virotherapy, becomes more important, and this book provides a thorough resource for this area of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Vaccinia, Mpox, and Other Poxviruses: Methods and Protocols serves as an ideal resource for virologists working in this vital section of the field.