Vaccine Technologies for Veterinary Viral Diseases

Methods and Protocols

Häftad, Engelska, 2023

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This second edition includes a collection of antigen production and delivery strategies for vaccine development in veterinary species.

An overview of veterinary viral diseases and vaccine technologies.- Production and purification of candidate subunit vaccines by IC-tagging protein encapsulation.- Elastin‐like polymers as nanovaccines: protein engineering of self‐assembled, epitope-exposing nanoparticles.- Display of heterologous proteins in Bacillus subtilis biofilms for enteric immunization.- Production of influenza H5 vaccine oligomers in plants.- DNA vaccines in pigs: from immunization to antigen identification.- Use of foot-and-mouth disease virus non-coding synthetic RNAs as vaccine adjuvants.- Evaluation of innate lymphoid cells and dendritic cells following viral vector vaccination.- Generation of replication deficient human adenovirus 5 (Ad5) vectored FMD vaccines.- Recombinant modified vaccinia virus Ankara development to express VP2, NS1, and VP7 proteins of Bluetongue virus.- Cloning strategies for the generation of recombinant capripoxvirus through the use of screening and selection markers.- Using Rift Valley fever virus as a vector platform for the expression of ruminant disease antigens.- Generation and characterization of single-cycle infectious canine Influenza A Virus (sciCIV) and its use as vaccine platform.- Reverse genetics for influenza A and B viruses driven by swine polymerase I promoter.- Analysis of the cellular immune responses to vaccines.