• Overview of Vaccines.- Temperature-Sensitive Mutant Vaccines.- Live Viral Vectors.- Live Viral Vectors.- Live Viral Vectors.- Development of Attenuated Salmonella Strains That Express Heterologous Antigens.- Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria.- Synthetic Peptides.- Genetic Detoxification of Bacterial Toxins.- Preparation of Polysaccharide-Conjugate Vaccines.- Adjuvant Formulations for Experimental Vaccines.- Incorporation of Immunomodulators into Plasmid DNA Vaccines.- Microencapsulation of Vaccine Antigens.- Lyophilization of Vaccines.- Stimulation of Mucosal Immunity.- Induction and Detection of T-Cell Responses.- Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses.- Assessment of Functional Antibody Responses.- The Use of Complete Genome Sequences in Vaccine Design.- Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment.- Clinical Trials.- Assuring the Quality and Safety of Vaccines.- DNA Vaccination.- From Vaccine Research to Manufacture.

"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMS From reviews of the first edition... "...cover[s] concepts and protocols applicable to a wide range of vaccines and infectious diseases and cancer...brings together many protocols of value to research and development scientists involved in the vaccine technology area." -Immunology and Cell Biology "...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation." -Doody's Health Sciences Book Review Journal

• Overview of Vaccines.- Temperature-Sensitive Mutant Vaccines.- Live Viral Vectors.- Live Viral Vectors.- Live Viral Vectors.- Development of Attenuated Salmonella Strains That Express Heterologous Antigens.- Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria.- Synthetic Peptides.- Genetic Detoxification of Bacterial Toxins.- Preparation of Polysaccharide-Conjugate Vaccines.- Adjuvant Formulations for Experimental Vaccines.- Incorporation of Immunomodulators into Plasmid DNA Vaccines.- Microencapsulation of Vaccine Antigens.- Lyophilization of Vaccines.- Stimulation of Mucosal Immunity.- Induction and Detection of T-Cell Responses.- Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses.- Assessment of Functional Antibody Responses.- The Use of Complete Genome Sequences in Vaccine Design.- Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment.- Clinical Trials.- Assuring the Quality and Safety of Vaccines.- DNA Vaccination.- From Vaccine Research to Manufacture.

"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMS From reviews of the first edition... "...cover[s] concepts and protocols applicable to a wide range of vaccines and infectious diseases and cancer...brings together many protocols of value to research and development scientists involved in the vaccine technology area." -Immunology and Cell Biology "...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation." -Doody's Health Sciences Book Review Journal

• Overview of Vaccines.- Temperature-Sensitive Mutant Vaccines.- Live Viral Vectors.- Live Viral Vectors.- Live Viral Vectors.- Development of Attenuated Salmonella Strains That Express Heterologous Antigens.- Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria.- Synthetic Peptides.- Genetic Detoxification of Bacterial Toxins.- Preparation of Polysaccharide-Conjugate Vaccines.- Adjuvant Formulations for Experimental Vaccines.- Incorporation of Immunomodulators into Plasmid DNA Vaccines.- Microencapsulation of Vaccine Antigens.- Lyophilization of Vaccines.- Stimulation of Mucosal Immunity.- Induction and Detection of T-Cell Responses.- Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses.- Assessment of Functional Antibody Responses.- The Use of Complete Genome Sequences in Vaccine Design.- Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment.- Clinical Trials.- Assuring the Quality and Safety of Vaccines.- DNA Vaccination.- From Vaccine Research to Manufacture.

"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMS From reviews of the first edition... "...cover[s] concepts and protocols applicable to a wide range of vaccines and infectious diseases and cancer...brings together many protocols of value to research and development scientists involved in the vaccine technology area." -Immunology and Cell Biology "...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation." -Doody's Health Sciences Book Review Journal