Del i serien Methods in Molecular Biology

Ubiquitin-Proteasome Protocols

Inbunden, Engelska, 2005

1 429 kr

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This volume is provides the tools for investigators to learn how to manipulate the ubiqutin-proteasome system to address questions in their own areas of reaserch. This borad field is covered in detail from basic biochemistry to cellular assays to discovery techniques using mass spectroscopic analysis. It will serve as a useful tool to accelerate discovery and enhance productivity.

Produktinformation

Utgivningsdatum2005-04-01
Mått155 x 235 x 26 mm
Vikt776 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor381
Upplaga2005
FörlagHumana Press Inc.
ISBN9781588292520

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik
Medicin: icke kliniska discipliner inom Medicin

Biochemical Methods to Study the Ubiquitin-Proteasome System.- Small-Molecule Inhibitors of Proteasome Activity.- Purification of E1 and E1-Like Enzymes.- Assays for RING Family Ubiquitin Ligases.- Ubiquitin Chain Synthesis.- Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast.- Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome.- Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation.- Assays of Proteasome-Dependent Cleavage Products.- Identification of Components of Protein Complexes.- Mass Spectrometric Determination of Protein Ubiquitination.- Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol.- Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation.- Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization.- Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions.- Measuring Ubiquitin Conjugation in Cells.- Assays for Proteasome Assembly and Maturation.- N-Terminal Ubiquitination.- Quantitating Defective Ribosome Products.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells.- Aggresome Formation.- Detection of Sumoylated Proteins.- Proteasome Inhibitors in Cancer Therapy.- Parkinson’s Disease.