Ubiquitin-dependent Protein Degradation

Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental BiologyYale University, USA.

• 1. Assays for protein retrotranslocation in ERADSonya Neal, Sascha H. Duttke and Randolph Y. Hampton2. Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiaeRahul S. Samant and Judith Frydman3. Methodologies to monitor protein turnover at the inner nuclear membrane Pei-Ling Tsai, Chenguang Zhao and Christian Schlieker4. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 Bayan Mashahreh, Yuval Reiss, Reuven Wiener and Tommer Ravid5. Methods for genetic analysis of mammalian ER-associated degradationDara E. Leto and Ron R. Kopito6. A dual system to manipulate protein levels for DNA replication- and cell cycle-related studiesNéstor García-Rodríguez and Helle D. Ulrich7. Engineered disulfide crosslinking to measure conformational changes in the 26S proteasomeRandi G. Reed and Robert J. Tomko Jr.8. Assays for ubiquitin-like protein ligation and proteasome function in archaeaXian Fu, Zachary Adams and Julie Maupin-Furlow9. Structural mass spectrometry approaches to study the 20S proteasomeGili Ben-Nissan, Shay Vimer, Mark Tarnavsky and Michal Sharon10. Single-molecule methods for measuring ubiquitination and protein stabilityJason Hon and Ying Lu11. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylationSrinivasan Muniyappan and Byung-Hoon Lee12. Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathwayEvan L. Guiney, Lu Zhu, Richa Sardana, Scott D. Emr and Matthew G. Baile13. Detection of ubiquitinated targets in mammalian and Drosophila modelsElena Maspero, Valentina Fajner, Janine Weber and Simona Polo14. Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegansAlexander Springhorn and Thorsten Hoppe15. Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cellsVictoria Cohen-Kaplan, Ido Livneh, Yong Tae Kwon and Aaron Ciechanover

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