Tumor Microenvironment

Study Protocols

Häftad, Engelska, 2018

Av Constantinos Koumenis, Lisa M. Coussens, Amato Giaccia, Ester Hammond

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This volume covers the topics presented at the 3rd International Conference on Tumor Microenvironment and Cellular Stress by an international community of researchers.

Produktinformation

Utgivningsdatum2018-05-30
Mått155 x 235 x 17 mm
Vikt470 g
FormatHäftad
SpråkEngelska
SerieAdvances in Experimental Medicine and Biology
Antal sidor297
FörlagSpringer International Publishing AG
ISBN9783319799933

Tillhör följande kategorier

Onkologi inom Medicin
Anatomi inom Medicin
Biologi inom Naturvetenskap och teknik
Medicinsk utrustning och medicinska tekniker inom Medicin

1.Staining against phospho-H2AX (γ-H2AX) as a marker for DNA damage and genomic instability in cancer tissues and cells.- 2.Measuring DNA replication rates in hypoxic conditions.- 3.Isolation of Proteins on Nascent DNA in hypoxia and reoxygenation conditions.- 4.In vivo interrogation of the hypoxic transcriptome of solid tumors: optimizing hypoxic probe labelling with Laser-Capture Microdissection for isolation of high-quality RNA for deep sequencing analysis.- 5.Gas chromatography coupled to mass spectrometry (GC-MS) to study metabolism in cultured cells.- 6.Probing Cancer Cell Metabolism using NMR Spectroscopy.- 7.Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenases Activity in Cultured Cells.- 8.Measuring Autophagy in the Context of Cancer.- 9.Methods for studying autophagy within the tumor microenvironment.- 10.Using 3 Dimensional Culture (Spheroids) as an in vitro Model of Tumour Hypoxia.- 11.Isolation of Glioma Initiating Cells for Biological Study.- 12.Isolation of mouse and human tumor associated macrophages.- 13.Mouse models of brain metastasis for unravelling tumour progression.- 14.Quantification of lung metastases from in vivo mouse models.- 15.A multimodal data analysis approach for targeted drug discovery involving topological data analysis (TDA).- 16.Efficient protocol for the identification of hypoxic cell radiosensitisers.