Tumor Microenvironment

Methods and Protocols

Häftad, Engelska, 2018

1 549 kr

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This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis.

Produktinformation

Utgivningsdatum2018-06-09
Mått178 x 254 x 20 mm
Vikt692 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor356
FörlagHumana Press Inc.
ISBN9781493981434

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Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible.- Laser Capture Microdissection as a Tool to Study Tumor Stroma.- Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging.- Studies on the Tumor Vasculature and Coagulant Microenvironment.- A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment.- Measuring Vascular Permeability In Vivo.- Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL).- Analyzing the Tumor Microenvironment by Flow Cytometry.- Detecting Secreted Analytes from Immune Cells: An Overview of Technologies.- Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting.- Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.- Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies.- Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer.- Analysis of Extracellular Vesicles in the Tumor Microenvironment.- Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disc Confocal Microscopy.- Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice.- Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer.- Modeling Breast Tumor Development with a Humanized Mouse Model.- CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice.- Metabolomics Analyses of Cancer Cells in Controlled Microenvironments.- Analysis of the Tumor Microenvironment Transcriptome Via NanoString mRNA and miRNA Expression Profiling.- RNA-Seq as a Tool to Study the Tumor Microenvironment.- Sample Preparation for MassSpectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues.