Transgenic Plants

Methods and Protocols

Inbunden, Engelska, 2018

AvSandeep Kumar,Pierluigi Barone,Michelle Smith

2 689 kr

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This book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments.

Produktinformation

Utgivningsdatum2018-11-11
Mått178 x 254 x 33 mm
Vikt1 020 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor444
FörlagHumana Press Inc.
ISBN9781493987771

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Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och natur

Repurposing macromolecule delivery tools for plant genetic modification in the era of precision genome engineering.- The use of an automated platform to assemble multigenic constructs for plant transformation.- Ensifer-mediated transformation (emt) of rice (monocot) and oilseed rape (dicot).- Setaria viridis as a model plant for functional genomic studies in C4 crops.- Transient transformation using particle bombardment for gene expression analysis.- Maize transformation using the morphogenic genes babyboom and wuschel2.- Efficient and fast production of transgenic rice plants by agrobacterium-mediated transformation.- Protocol for Agrobacterium-mediated transformation and transgenic plant production of switchgrass.- Biolistic transformation of wheat.- Mesophyll protoplasts and PEG-mediated transfections: Transient assays and generation of stable transgenic canola plants.- A Unified Agrobacterium-mediated transformation protocol for alfalfa (Medicago sativa l.) and medicago truncatula.- Poplar transformation.- The genetic transformation of sweet orange (Citrus sinensis L. Osbeck) for enhanced resistance to citrus canker.- Genetic modification of grapevine embryogenic culture.- Agrobacterium-mediated transformation of Solanum tuberosum L., potato.- Agrobacterium tumefaciens-mediated transformation of tomato.- DNA break repair in plants and its application for genome engineering.- Gene stacking in plants through the application of site-specific recombination and nuclease activity.- CRISPR/Cas9 for mutagenesis in rice.- Plant biotechnology applications of zinc fingers technology.- Overview of biotechnology-derived herbicide tolerance and insect resistance traits in plant agriculture.- Developing transgenic agronomic traits for crops: targets, methods and challenges.- Transgenic and genome editing approaches for modifying plant oils.- Summary of molecularanalysis methods for characterizing transgenic events.- Detection of transgenic proteins by immunoassays.- Systematic evaluation of field crop performance using modern phenotyping tools and techniques.