Therapeutic Oligonucleotides

• Therapeutic Oligonucleotides.- Dinucleotides Containing 3¢-S-Phosphorothiolate Linkages.- 2’-O,4’-C-Methyleneoxymethylene Bridged Nucleic Acids (2’,4’-BNACOC).- A Non-covalent Peptide-based Strategy for Ex-vivo and In vivo Oligonucleotide Delivery.- Cell Penetrating Peptides-based Strategies for the Delivery of Splice Redirecting Antisense Oligonucleotides.- A Nanoparticle for Tumor Targeted Delivery of Oligomers.- Light-directed Delivery of Nucleic Acids.- Antibody Targeted siRNA Delivery.- Aptamer-Drug Conjugation for Targeted Tumor Cell Therapy.- Five-step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2.- Diverse Small Non-coding RNAs in RNA Interference Pathways.- Quantification of siRNAs In vitro and In vivo.- Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.- siRNA Knockdown of Gene Expression in Endothelial Cells.- Using RNA Interference in Schistosoma mansoni.- Performing the Labeled microRNA Pull-down (LAMP) Assay System: An Experimental Approach for High-throughput Identification of microRNA-target mRNAs.- Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8.- Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-like Receptor 9.- Surface Plasmon Resonance Investigation of RNA Aptamer-RNA Ligand Interactions.- Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein.- Inhibition of Human Papillomavirus Expression Using DNAzymes

From the reviews: "The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research scientists interested in this area. ... This is an extremely useful resource that provides different ways that oligonucleotides may be applied as therapeutics. Very few books cover the field as thoroughly and as clearly as this one." (Omer Iqbal, Doody's Book Reviews, November, 2011)