Del 0 - Methods in Molecular Biology

Therapeutic Antibodies

Methods and Protocols

Inbunden, Engelska, 2021

2 249 kr

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This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects.

Produktinformation

Utgivningsdatum2021-09-04
Mått178 x 254 x undefined mm
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor347
FörlagSpringer-Verlag New York Inc.
ISBN9781071614495

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Farmakologi inom Medicin
Naturvetenskap:allmänt inom Naturvetenskap och teknik

Therapeutic Antibodies: An Overview.- Treatment of Autoimmune Diseases with Therapeutic Antibodies: Lessons Learned from PID Patients Allow for Stratification of the Infection Risk.- B-Cell Analysis for Monitoring Patients Undergoing B-Cell Depletion for the Treatment of Autoimmune Diseases.- Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods.- The Therapeutic Antibody Profiler for Computational Developability Assessment.- Short Read-Length Next-Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs.- Large Scale Transient Production in ExpiCHO-STM with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection.- Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris.- Purification of Therapeutic Antibodies by Protein A Affinity Chromatography.- Ion Exchange Chromatographic Methods for Purification of Therapeutic Antibodies.- Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies.- Use of PASEF for Accelerated Protein Sequence Confirmation and De Novo Sequencing with High Data Quality.- Nano-Microscopy of Therapeutic Antibody Aggregates in Solution.- Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering.- Intact Mass Quantitation of Therapeutic Antibodies for Pharmacokinetic Studies Using Immuno-Purification.- Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS.- Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies.- Methods for Functional Characterization of FcRn Interactions with Therapeutic Antibodies and Fc-Fusion Proteins.- Method for Measurement of Antibody-Dependent Cellular Phagocytosis.- Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection.- A Surface Plasmon Resonance-Based Assay for Simultaneous Measurement of Serum Concentrations of Therapeutic Antibodies and Anti-Drug Antibodies.