TGF-β Signaling

Methods and Protocols

Häftad, Engelska, 2016

1 569 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

Chapters provide basic introduction of TGF-ß signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-ß signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway;

Produktinformation

Utgivningsdatum2016-08-23
Mått178 x 254 x 23 mm
Vikt764 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor394
FörlagHumana Press Inc.
ISBN9781493949939

Tillhör följande kategorier

Cellbiologi inom Naturvetenskap och teknik

Regulation of TGF-b Receptors.- Determining TGF-β Receptor Levels in the Cell Membrane.- Post-translational Modifications of TGF-b Receptors.- Production, Isolation，and Structural Analysis of Ligands and Receptors of the TGF-b Superfamily.- Phosphorylation of Smads by Intracellular Kinases.- Analysis of Smad Phosphatase Activity in vitro.- Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGF-b Signaling.- TGF-β Signaling in Stem Cell Regulation.- Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β.- In vitro Th differentiation protocol.- Interrogating TGF-b Function and Regulation in Endothelial Cells.- Isolation and Manipulation of Adipogenic Cells to Assess TGF-b Superfamily Functions.- Imaging TGFb Signaling in Mouse Models of Cancer Metastasis.- Generation and Characterization of Smad7 Conditional Knockout Mice.- Monitoring Smad Activity in vivo Using the Xenopus Model System.- Animal Cap Assay for TGF-b Signaling.- Detection of Smad Signaling in Zebrafish Embryos.- Role of TGF-β signaling in Coupling Bone Remodeling.- Studying the Functions of TGF-β Signaling in the Ovary.- Quantitative Real-Time PCR Analysis of MicroRNAs and Their Precursors Regulated byTGF-b Signaling.- TGF-β Regulated microRNAs and Their Function in Cancer Biology.- Epigenomic Regulation Of Smad1 Signaling During Cellular Senescence Induced by Ras Activation.- The Role of Ubiquitination to Determine Non-Smad Signaling Responses.- Genome-wide RNAi Screening to Dissect the TGF-b Signal Transduction Pathway.- Measuring TGF-β Ligand Dynamics in Culture Medium.